Atlanta United 2 | Birmingham Legion | Charleston Battery | Detroit City FC | Hartford Athletic | Indy Eleven | Loudoun United | Louisville City | Memphis 901 FC | Miami FC | New York Red Bulls II | Pittsburgh Riverhounds | Tampa Bay Rowdies | FC Tulsa

Colorado Springs Switchbacks | El Paso Locomotive | LA Galaxy II | Las Vegas Lights | Monterey Bay FC | New Mexico United | Oakland Roots | Orange County SC | Phoenix Rising | Rio Grande Valley Toros | Sacramento Republic | San Antonio FC | San Diego Loyal

Austin Bold (Rückkehr 2023) | Oklahoma City Energy (Rückkehr 2023)

Buffalo (2023) | Des Moines (2024) | Queensboro FC (2023) | Rhode Island (2023)

Antigua Barracuda (2011–2013) | Austin Aztex (2011–2017) | Bethlehem Steel/Philadelphia Union II (2016–2020) | Carolina RailHawks/North Carolina FC (2018–2020) | Charlotte Eagles (2011–2014) | Charlotte Independence (2015–2021) | FC Cincinnati (2015–2018) | Dayton Dutch Lions (2011–2014) | FC Montréal (2015–2016) | FC New York (2011) | Harrisburg City Islanders/Penn FC (2011–2018) | Orlando City (2011–2014) | Ottawa Fury (2017–2019) | Phoenix FC (2013) | Portland Timbers 2 (2015–2020) | FC Sevilla Puerto Rico (2011) | Puerto Rico United (2011) | Real Monarchs (2015–2021) | Reno 1868 FC (2017–2020) | River Plate Puerto Rico (2011) | Richmond Kickers (2011–2018) | Rochester Rhinos (2011–2017) | Saint Louis FC (2015–2020) | Seattle Sounders 2/Tacoma Defiance (2015–2021) | Swope Park Rangers/Sporting Kansas City II (2016–2021) | Toronto FC II (2011–2018) | Vancouver Whitecaps 2 (2015–2017) | Tampa Bay FC (2013) | Wilmington Hammerheads (2011–2016)